Grandl, Margot and Bared, Salim Maa and Liebisch, Gerhard and Werner, Tobias and Barlage, Stefan and Schmitz, Gerd (2006) E-LDL and Ox-LDL differentially regulate ceramide and cholesterol raft microdomains in human Macrophages. Cytometry. Part A : the journal of the International Society for Analytical Cytology 69 (3), pp. 189-191.
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Other URL: http://dx.doi.org/10.1002/cyto.a.20232
Atherosclerosis is characterized by the generation of lipid-loaded macrophage-derived foam cells. To study the effect of different types of atherogenic lipoproteins, human macrophages were loaded with enzymatically degraded low density lipoprotein (E-LDL) or oxidized LDL (Ox-LDL). Cellular cholesterol content was increased by E-LDL, whereas Ox-LDL increased the ceramide content. Cell surface expression analysis by flow cytometry and confocal microscopy revealed that Ox-LDL increased ceramide and lactosylceramide expression compared to E-LDL loading and induced ceramide rafts, whereas loading with E-LDL induced cholesterol-rich microdomains. Formation of different rafts may have consequences for raft associated signaling in cholesterol homeostasis and apoptosis in human macrophages.
|Institutions:||Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||01 Mar 2007|
|Last Modified:||20 Jul 2011 20:51|