Barlage, Stefan and Boettcher, Dorothee and Boettcher, Alfred and Dada, Ashraf and Schmitz, Gerd (2006) High density lipoprotein modulates platelet function. Cytometry. Part A : the journal of the International Society for Analytical Cytology 69 (3), pp. 196-199.
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Other URL: http://dx.doi.org/10.1002/cyto.a.20214
BACKGROUND: Platelet activation by atherogenic lipoproteins can be antagonized by high density lipoprotein (HDL), probably via interaction with the ATP-binding cassette transporter A1 (ABCA1). METHODS: ABCA1 expression and its association with cholesterol rich membrane domains was analyzed by mRNA and Western blot analysis. HDL effects on platelet receptor clustering were analyzed by flow cytometric analysis of fluorescence resonance energy transfer between fluorochrome-labeled antibodies. RESULTS: ABCA1 expression increased upon megakaryocytic differentiation of human stem cells and ABCA1 protein partially associated to LubroIWX-resistant membrane domains. Plasma HDL-cholesterol in healthy donors negatively correlated to the platelet membrane cholesterol content. Receptor cluster analysis revealed a decrease in the association of Gplb and FcgammaRII upon incubation of platelets with HDL3. CONCLUSION: Our results suggest that HDL modulates platelet reactivity by altering lipid raft associated receptor clustering.
|Institutions:||Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin|
|Keywords:||HDL; platelet; ABCA1; membrane raft|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||08 Feb 2007|
|Last Modified:||20 Jul 2011 20:51|