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Reconstitution of a PolII-like RNA polymerase and contribution of subunit E� and structural elements in the active center to RNA polymerase functions

URN to cite this document: urn:nbn:de:bvb:355-opus-7644

Naji, Souad (2007) Reconstitution of a PolII-like RNA polymerase and contribution of subunit E� and structural elements in the active center to RNA polymerase functions. PhD, Universität Regensburg

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Abstract (English)

The main focus of the present work was the functional and structural characterization of Pyrococcus furiosus RNAP. A protocol was developed for the purification of the endogenous RNAP to homogeneity and in a large scale. Transmission electron microscope images of this purified denzyme show that the archaeal Pfu RNAP can adopt different forms and it is slightly elongated in shape with a diameter ...

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Translation of the abstract (German)

Das Hauptthema meiner Doktorarbeit war eine Analyse von Struktur-Funktionsbeziehungen innerhalb der Pyrococcus furiosus (Pfu) RNA Polymerase (RNAP). Ein Verfahren für die Reinigung der endogenen RNAP im großen Maßstab wurde etabliert. Transmissionselektronenmikroskopische Aufnahmen des Enzyms wiesen nach, dass die Pfu RNAP verschiedene Formen haben kann, die einen Durchmesser von etwa 13-15 nm ...

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Item Type:Thesis of the University of Regensburg (PhD)
Date:16 July 2007
Referee:Michael (Prof. Dr.) Thomm
Date of exam:21 December 2006
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Michael Thomm
Keywords:Archaeabakterien , Transkription <Genetik> , RNS-Polymerase II , Untereinheit , Struktur-Aktivitäts-Beziehung , Pyrococcus furiosus , , Archaea , transcription , RNA polymerase , subunit E� , active center
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:27 Oct 2009 11:53
Last Modified:13 Mar 2014 11:45
Item ID:10543
Owner Only: item control page

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