Hintz, M. and Reichenberg, A. and Altincicek, B. and Bahr, U. and Gschwind, R. M. and Kollas, A. and Beck, E. and Wiesner, J. and Eberl, M. and Jomaa, H. (2001) Identification of 4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gamma delta T cells in Escherichia coli. FEBS Letters 509 (2), pp. 317-322.
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The gcpE and lytB gene products control the terminal steps of isoprenoid biosynthesis via the 2-C-methyl-Image -erythritol 4-phosphate pathway in Escherichia coli. In lytB-deficient mutants, a highly immunogenic compound accumulates significantly, compared to wild-type E. coli, but is apparently absent in gcpE-deficient mutants. Here, this compound was purified from E. coli ΔlytB mutants by preparative anion exchange chromatography, and identified by mass spectrometry, 1H, 13C and 31P NMR spectroscopy, and NOESY analysis as (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP). HMB-PP is 104 times more potent in activating human Vγ9/Vδ2 T cells than isopentenyl pyrophosphate.
|Institutions:||Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Prof. Dr. Ruth Gschwind|
|Keywords:||γδ T cell; 2-C-Methyl-Image -erythritol 4-phosphate pathway; 4-Hydroxy-3-methyl-but-2-enyl pyrophosphate; gcpE; lytB|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Deposited On:||11 Nov 2009 17:12|
|Last Modified:||11 Nov 2009 17:12|