Maisch, Tim and Wagner, J and Papastamou, V and Nerl, H-J and Hiller, Karl-Anton and Szeimies, Rolf-Markus and Schmalz, Gottfried (2009) Combination of 10% EDTA, Photosan, and a blue light hand-held photopolymerizer to inactivate leading oral bacteria in dentistry in vitro. Journal of applied microbiology 107 (5), pp. 1569-1578.
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AIMS: The goal of this study was to investigate the phototoxicity of Photosan in combination with EDTA and a hand-held photopolymerizer used in dentistry for light-curing resins against leading key pathogens in caries, endodontic treatment failures, and periodontitis respectively. METHODS AND RESULTS: Cellular uptake of Photosan was detected by fluorescence spectroscopy for Streptococcus mutans and Enterococcus faecalis but not for Aggregatibacter actinomycetemcomitans. Addition of 10% EDTA enabled the uptake of Photosan by A. actinomycetemcomitans. Killing of S. mutans and E. faecalis mediated by Photosan and blue light was concentration and light dose dependent, achieving a >or=99.9% (>or=3 log(10) reduction) efficacy of bacteria killing. In the presence of 10% EDTA, Photosan induced a reduction of >or=4 log(10) in the viability of A. actinomycetemcomitans at a concentration of 50 microg ml(-1), upon activation at a dose of 9.65 J cm(-2) for 60 s. EDTA alone, light alone, and Photosan alone were not able to kill bacteria. CONCLUSIONS: Ten per cent EDTA and Photosan cause a potent phototoxicity against oral bacteria upon illumination with a photopolymerizer. SIGNIFICANCE AND IMPACT OF THE STUDY: Increasing antibiotic resistance and insufficient drug concentrations within the sulcus fluid are responsible for lacking antimicrobial efficacy. This study provides useful information that combination of Photosan, EDTA, and a photopolymerizer may be a potentially powerful tool for the efficient destroying of key oral bacteria.
|Institutions:|| Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie|
Medicine > Lehrstuhl für Dermatologie und Venerologie
Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||03 Nov 2009 10:26|
|Last Modified:||20 Jul 2011 22:06|