Deppenmeier, Uwe and Johann, André and Hartsch, Thomas and Merkl, Rainer and Schmitz, Ruth A: and Martinez-Arias, Rosa and Henne, Anke and Wiezer, Arnim and Bäumer, Sebastian and Jacobi, Carsten and Brüggemann, Holger and Lienard, Tanja and Christmann, Andreas and Bömeke, Mechthild and Steckel, Silke and Bhattacharyya, Anamitra and Lykidis, Athanasios and Overbeek, Ross and Klenk, Hans Peter and Gunsalus, Robert P. and Fritz, Hans Joachim and Gottschalk, Gerhard (2002) The genome of Methanosarcina mazei: evidence for lateral gene transfer between bacteria and archaea. Journal of Molecular Microbiology and Biotechnology 4 (4), pp. 453-461.
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The Archaeon Methanosarcina mazei and related species are of great ecological importance as they are the only organisms fermenting acetate, methylamines and methanol to methane, carbon dioxide and ammonia (in case of methylamines). Since acetate is the precursor of 60% of the methane produced on earth these organisms contribute significantly to the production of this greenhouse gas, e.g. in rice paddies. The 4,096,345 base pairs circular chromosome of M. mazei is more than twice as large as the genomes of the methanogenic Archaea currently completely sequenced (Bult et al., 1996; Smith et al., 1997). 3,371 open reading frames (ORFs) were identified. Based on currently available sequence data 376 of these ORFs are Methanosarcina-specific and 1,043 ORFs find their closest homologue in the bacterial domain. 544 of these ORFs reach significant similarity values only in the bacterial domain. They include 56 of the 102 transposases, and proteins involved in gluconeogenesis, proline biosynthesis, transport processes, DNA-repair, environmental sensing, gene regulation, and stress response. Striking examples are the occurrence of the bacterial GroEL/GroES chaperone system and the presence of tetrahydrofolate-dependent enzymes. These findings might indicate that lateral gene transfer has played an important evolutionary role in forging the physiology of this metabolically versatile methanogen.
|Institutions:||Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner > Arbeitsgruppe PD Dr. Rainer Merkl|
|Keywords:||Archaea/*genetics Bacteria/classification/*genetics Gene Transfer Techniques *Genome, Archaeal Methanosarcina/classification/*genetics/metabolism Open Reading Frames Phylogeny Support, Non-U.S. Gov't|
|Subjects:||500 Science > 570 Life sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Deposited On:||18 Nov 2009 09:16|
|Last Modified:||21 Dec 2009 07:46|