| Dokumentenart: | Artikel | ||||||||||||||||||||||||
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| Titel eines Journals oder einer Zeitschrift: | The Journal of Biological Chemistry | ||||||||||||||||||||||||
| Verlag: | American Society for Biochemistry and Molecular Biology | ||||||||||||||||||||||||
| Band: | 262 | ||||||||||||||||||||||||
| Nummer des Zeitschriftenheftes oder des Kapitels: | 34 | ||||||||||||||||||||||||
| Seitenbereich: | S. 16618-16624 | ||||||||||||||||||||||||
| Datum: | 1987 | ||||||||||||||||||||||||
| Institutionen: | Biologie und Vorklinische Medizin > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl | ||||||||||||||||||||||||
| Identifikationsnummer: |
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| Klassifikation: |
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| Stichwörter / Keywords: | Antibodies, Monoclonal Biological Transport, Active Carbohydrates/analysis Concanavalin A/metabolism Dictyostelium/*analysis Fluorescent Antibody Technique Glycosylation Molecular Weight Support, Non-U.S. Gov't Surface Properties Viral Proteins/*metabolism Wheat Germ Agglutinins/metabolism | ||||||||||||||||||||||||
| Dewey-Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie | ||||||||||||||||||||||||
| Status: | Veröffentlicht | ||||||||||||||||||||||||
| Begutachtet: | Ja, diese Version wurde begutachtet | ||||||||||||||||||||||||
| An der Universität Regensburg entstanden: | Nein | ||||||||||||||||||||||||
| Dokumenten-ID: | 10916 |
Zusammenfassung
Two different types of oligosaccharides, designated type 1 and 2 carbohydrate residues, are present on the contact site A molecule, an 80-kDa glycoprotein involved in the formation of EDTA-stable cell adhesion during cell aggregation in Dictyostelium discoideum. The first precursor detected by pulse-chase labeling with [35S]methionine was a 68-kDa glycoprotein carrying type 1 carbohydrate. ...
Zusammenfassung
Two different types of oligosaccharides, designated type 1 and 2 carbohydrate residues, are present on the contact site A molecule, an 80-kDa glycoprotein involved in the formation of EDTA-stable cell adhesion during cell aggregation in Dictyostelium discoideum. The first precursor detected by pulse-chase labeling with [35S]methionine was a 68-kDa glycoprotein carrying type 1 carbohydrate. Conversion of the precursor into the 80-kDa form occurred simultaneously with the addition of type 2 carbohydrate. Tunicamycin inhibited type 1 glycosylation more efficiently than type 2 glycosylation. The first precursor detected in tunicamycin-treated cells by pulse-chase labeling was a 53-kDa protein lacking both carbohydrates, which was converted through addition of type 2 carbohydrate into a 66-kDa final product. Labeling of intact cells indicated that this 66-kDa glycoprotein is transported to the cell surface. Prolonged treatment with tunicamycin resulted in the accumulation within the cells of the 53-kDa precursor with no detectable exposure of this protein on the cell surface. It is concluded that type 1 carbohydrate, which is cotranslationally added in N-glycosidic linkages, is neither required for transport of the protein to the Golgi apparatus nor for type 2 glycosylation or protection of the protein against proteolytic degradation. Incapability of tunicamycin-treated cells of forming EDTA-stable cell contacts suggests a role for type 1 carbohydrate in cell adhesion. Type 2 carbohydrate is added posttranslationally. It is required in the absence of type 1 glycosylation for transport of the protein to the cell surface.
Metadaten zuletzt geändert: 24 Mai 2018 11:42
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