Larbig, Karen D. and Christmann, Andreas and Johann, André and Klockgether, Jens and Hartsch, Thomas and Merkl, Rainer and Wiehlmann, Lotz and Fritz, Hans Joachim and Tümmler, Burkhard
Gene Islands Integrated into tRNA(Gly) Genes Confer Genome Diversity on a Pseudomonas aeruginosa Clone.
Journal of Bacteriology 184 (23), pp. 6665-6680.
Other URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC135438/?tool=pubmed
Intraclonal genome diversity of Pseudomonas aeruginosa was studied in one of the most diverse mosaic regions of the P. aeruginosa chromosome. The ca. 110-kb large hypervariable region located near the lipH gene in two members of the predominant P. aeruginosa clone C, strain C and strain SG17M, was sequenced. In both strains the region consists of an individual strain-specific gene island of 111 (strain C) or 106 (SG17M) open reading frames (ORFs) and of a 7-kb stretch of clone C-specific sequence of 9 ORFs. The gene islands are integrated into conserved tRNA(Gly) genes and have a bipartite structure. The first part adjacent to the tRNA gene consists of strain-specific ORFs encoding metabolic functions and transporters, the majority of which have homologs of known function in other eubacteria, such as hemophores, cytochrome c biosynthesis, or mercury resistance. The second part is made up mostly of ORFs of yet-unknown function. Forty-seven of these ORFs are mutual homologs with a pairwise amino acid sequence identity of 35 to 88% and are arranged in the same order in the two gene islands. We hypothesize that this novel type of gene island derives from mobile elements which, upon integration, endow the recipient with strain-specific metabolic properties, thus possibly conferring on it a selective advantage in its specific habitat.
|Institutions:|| Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner > Arbeitsgruppe PD Dr. Rainer Merkl|
|Leukemia Inhibitory Factor||MESH|
|Molecular Sequence Data||MESH|
|Open Reading Frames||MESH|
|RNA, Transfer, Amino Acyl/genetics*||MESH|
|Sequence Analysis, DNA||MESH|
|Keywords:||Bacterial Proteins/genetics/metabolism Base Sequence Chromosome Mapping Codon Cosmids/genetics Cystic Fibrosis/microbiology *Genome, Bacterial Human Molecular Chaperones/genetics Molecular Sequence Data Open Reading Frames Pseudomonas aeruginosa/genetics/*pathogenicity RNA, Transfer, Amino Acyl/*genetics Sequence Analysis, DNA Species Specificity Support, Non-U.S. Gov't *Variation (Genetics)|
|Subjects:||500 Science > 570 Life sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Deposited On:||16 Nov 2009 10:30|
|Last Modified:||20 Jul 2011 22:07|