Veith, Birgit and Herzberg, Christina and Steckel, Silke and Feesche, Jörg and Maurer, Karl Heinz and Ehrenreich, Petra and Bäumer, Sebastian and Henne, Anke and Liesegang, Heiko and Merkl, Rainer and Ehrenreich, Armin and Gottschalk, Gerhard (2004) The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential. Journal of Molecular Microbiology and Biotechnology 7 (4), pp. 204-211.
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The genome of Bacillus licheniformis DSM13 consists of a single chromosome that has a size of 4,222,748 base pairs. The average G+C ratio is 46.2%. 4,286 open reading frames, 72 tRNA genes, 7 rRNA operons and 20 transposase genes were identified. The genome shows a marked co-linearity with Bacillus subtilis but contains defined inserted regions that can be identified at the sequence as well as at the functional level. B. licheniformis DSM13 has a well-conserved secretory system, no polyketide biosynthesis, but is able to form the lipopeptide lichenysin. From the further analysis of the genome sequence, we identified conserved regulatory DNA motives, the occurrence of the glyoxylate bypass and the presence of anaerobic ribonucleotide reductase explaining that B. licheniformis is able to grow on acetate and 2,3-butanediol as well as anaerobically on glucose. Many new genes of potential interest for biotechnological applications were found in B. licheniformis; candidates include proteases, pectate lyases, lipases and various polysaccharide degrading enzymes.
|Institutions:||Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner > Arbeitsgruppe PD Dr. Rainer Merkl|
|Keywords:||Bacillus/*genetics Bacillus subtilis/genetics Base Composition Biological Transport/genetics Chromosomes, Bacterial/*genetics DNA, Bacterial/*chemistry Endopeptidases/genetics Genes, Bacterial/genetics/physiology Genes, rRNA *Genome, Bacterial Genomics Glyoxylates/metabolism Lipase/genetics Lipoproteins/genetics Metabolism/genetics Molecular Sequence Data Open Reading Frames Peptides, Cyclic/genetics Polysaccharide-Lyases/genetics RNA, Transfer/genetics Recombination, Genetic Regulatory Sequences, Nucleic Acid Research Support, Non-U.S. Gov't Ribonucleotide Reductases/genetics *Sequence Analysis, DNA Synteny Transposases/genetics|
|Subjects:||500 Science > 570 Life sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Deposited On:||13 Nov 2009 11:03|
|Last Modified:||20 Jul 2011 22:07|