Schuierer, Marion M. and Heilmeier, Ursula and Boettcher, Alfred and Ugocsai, Peter and Bosserhoff, Anja K. and Schmitz, Gerd and Langmann, Thomas (2006) Induction of Raf kinase inhibitor protein contributes to macrophage differentiation. Biochemical and biophysical research communications 342 (4), pp. 1083-1087.
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Other URL: http://dx.doi.org/10.1016/j.bbrc.2006.02.083
Differential gene expression analysis of human blood monocytes has identified the Raf kinase inhibitor protein (RKIP) as a continuously upregulated gene in macrophage and dendritic cell maturation. Using realtime RT-PCR and Western blot analysis we were able to confirm the initial DNA-microarray findings of RKIP induction on mRNA and protein levels. RKIP upregulation in primary cells and overexpression in THP-1 cells did not alter ERK activity but strongly reduced the amount of the NFkappaB subunit p65 in the nucleus. mRNA levels and cell surface expression of maturation markers including the integrin CD11c and the scavenger receptor CD36 were significantly increased in RKIP transfected THP-1 cells. Our data show for the first time that RKIP is upregulated during macrophage and dendritic cell differentiation on mRNA and protein levels and we conclude that RKIP contributes to the monocytic differentiation process via inhibition of the NFkappaB signaling cascade independent from the canonical Ras/Raf/MEK/ERK pathway.
|Date:||21 April 2006|
|Institutions:||Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin|
|Keywords:||Gene expression; Raf kinase inhibitor protein; Macrophage differentiation; NFκB; Scavenger receptors; THP-1 cells|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||01 Mar 2007|
|Last Modified:||20 Jul 2011 20:51|