Dittmar, Michael S. and Vatankhah, Bijan and Fehm, Nando P. and Schuierer, Gerhard and Bogdahn, Ulrich and Horn, Markus and Schlachetzki, Felix (2006) Fischer-344 rats are unsuitable for the MCAO filament model due to their cerebrovascular anatomy. Journal of neuroscience methods 156 (1-2), pp. 50-54.
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Middle cerebral artery occlusion (MCAO) in Fischer-344 rats results in a small variance of infarct size. However, complications are frequent especially in aged Fisher-344 rats undergoing endovascular suture occlusion of the middle cerebral artery. Analyzing our experiences with 165 Wistar, 13 Sprague-Dawley and 10 F-344 rats, we compared the incidence of impossible thread advancement and subarachnoid hemorrhage, respectively. Magnetic resonance angiography (MRA) was applied to study the course of the internal carotid artery (ICA) in Fischer and Wistar rats. Finally, we performed a structured review of the literature from 1991 to 2005 evaluating reports on Fischer rats subjected to intraluminal filament MCAO. Complications like fruitless filament advancement or subarachnoid hemorrhage were found to be significantly more frequent in Fischer rats than in other strains. MRA revealed significantly more pronounced kinking of the ICA in F-344 than in Wistar rats. In seven publications available on filament MCAO in F-344 rats, complication rates of 50-100% were reported, corroborating our data. Surgical difficulties accompanied by high complication rates due to their cerebrovascular anatomy make Fischer rats unsuitable for filament MCAO. If the use of Fischer rats for studies on focal cerebral ischemia is indicated, other ischemia models than intraluminal suture occlusion should be chosen.
|Date:||30 September 2006|
|Institutions:||Medicine > Lehrstuhl für Neurologie|
|Keywords:||Cerebral ischemia; Focal; Disease models; Animal; Rats; Middle cerebral artery occlusion; Carotid artery; Internal|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||02 Mar 2007|
|Last Modified:||20 Jul 2011 20:51|