Hecht, S. and Eisenreich, W. and Adam, P. and Amslinger, S. and Kis, K. and Bacher, A. and Arigoni, D. and Rohdich, F. (2001) Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein. Proc. Natl. Acad. Sci. USA 98 (26), pp. 14837-14842.
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Recombinant Escherichia coli cells engineered for the expression of the xyIB gene in conjunction with genes of the nonmevalonate pathway were supplied with C-13-labeled 1-deoxy-D-xylulose. Cell extracts were analyzed directly by NMR spectroscopy. C-13-labeled 2C-methyl-D-erythritol 2,4-cyclodiphosphate was detected at high levels in cells expressing xyIB, ispC, ispD, ispE, and ispF. The additional expression of the gcpE gene afforded 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate as an intermediate of the nonmevalonate pathway. Hypothetical mechanisms involving conserved cysteine residues are proposed for the enzymatic conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate catalyzed by the GcpE protein.
|Additional information (public):||Times Cited: 79|
|Institutions:||Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Dr. Sabine Amslinger|
|Keywords:||isoprenoid biosynthesis 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate Escherichia-coli isoprenoid biosynthesis isopentenyl diphosphate 4-diphosphocytidyl-2c-methyl-d-erythritol 1-deoxy-d-xylulose atoms|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Owner:||Dr. Sabine Amslinger|
|Deposited On:||24 Nov 2009 15:07|
|Last Modified:||13 Nov 2012 07:35|
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