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Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein

Hecht, S. and Eisenreich, W. and Adam, P. and Amslinger, S. and Kis, K. and Bacher, A. and Arigoni, D. and Rohdich, F. (2001) Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein. Proc. Natl. Acad. Sci. USA 98 (26), pp. 14837-14842.

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Abstract

Recombinant Escherichia coli cells engineered for the expression of the xyIB gene in conjunction with genes of the nonmevalonate pathway were supplied with C-13-labeled 1-deoxy-D-xylulose. Cell extracts were analyzed directly by NMR spectroscopy. C-13-labeled 2C-methyl-D-erythritol 2,4-cyclodiphosphate was detected at high levels in cells expressing xyIB, ispC, ispD, ispE, and ispF. The ...

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Item Type:Article
Date:2001
Additional information (public):Times Cited: 79
Institutions:Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Dr. Sabine Amslinger
Identification Number:
ValueType
ISI:000172848800021Web of Science ID
10.1073/pnas.201399298DOI
Keywords:isoprenoid biosynthesis 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate Escherichia-coli isoprenoid biosynthesis isopentenyl diphosphate 4-diphosphocytidyl-2c-methyl-d-erythritol 1-deoxy-d-xylulose atoms
Subjects:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:No
Owner: Dr. Sabine Amslinger
Deposited On:24 Nov 2009 14:07
Last Modified:13 Nov 2012 06:35
Item ID:11074
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