Hecht, S. and Kis, F. and Eisenreich, W. and Amslinger, S. and Wungsintaweekul, J. and Herz, S. and Rohdich, F. and Bacher, A. (2001) Enzyme-assisted preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate. J. Org. Chem. 66 (11), pp. 3948-3952.
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Recombinant l-deoxy-D-xylulose 5-phosphate synthase of Bacillus subtilis was used for the preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate using isotope-labeled glucose and/ or isotope-labeled pyruvate as starting materials. The simple one-pot methods described afford almost every conceivable isotopomer of l-deoxy-rt-xylulose 5-phosphate carrying C-13 Or C-14 from commercially available precursors with an overall yield around 50%.
|Additional information (public):||Times Cited: 16|
|Institutions:||Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Dr. Sabine Amslinger|
|Keywords:||Escherichia-coli biosynthesis precursor protein thiamin 4-diphosphocytidyl-2c-methyl-d-erythritol d-1-deoxyxylulose isoprenoids terpenoids catalyzes|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Owner:||Dr. Sabine Amslinger|
|Deposited On:||24 Nov 2009 14:24|
|Last Modified:||13 Nov 2012 06:34|