Rohdich, F. and Hecht, S. and Gärtner, K. and Adam, P. and Krieger, C. and Amslinger, S. and Arigoni, D. and Bacher, A. and Eisenreich, W. (2002) Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein. Proc. Natl. Acad. Sci. USA 99 (3), pp. 1158-1163.
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Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-C-13(5)]1-deoxy-D-xylulose into a 5:1 mixture of [U-C-13(5)]isopentenyl diphosphate and [U-C-13(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes.
|Additional information (public):||Times Cited: 94|
|Institutions:||Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Dr. Sabine Amslinger|
|Keywords:||biosynthesis of isoprenoids 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate isopentenyl diphosphate dimethylallyl diphosphate Isopentenyl-diphosphate isomerase escherichia-coli isoprenoid biosynthesis 2-c-methyl-d-erythritol 4-phosphate 4-(cytidine 5'-diphospho)-2-c-methyl-d-erythritol 5-phosphate precursor thiamin enzyme gene|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Owner:||Dr. Sabine Amslinger|
|Deposited On:||24 Nov 2009 15:20|
|Last Modified:||12 Nov 2012 14:55|
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