Göttler, Thomas and Holler, Eggehard (2006) Screening for β-poly(l-malate) binding proteins by affinity chromatography. Biochemical and Biophysical Research Communications 341 (4), pp. 1119-1127.
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Poly(β-l-malic acid) is a cell type-specific polymer of myxomycetes (true slime molds) with the physiological role to organize mobility of certain proteins over the giant multinucleated plasmodia. We have developed an affinity chromatography employing 1,6-diamino-n-hexane–Sepharose-coupled poly(malic acid) to identify such proteins in cellular extracts of Physarum polycephalum. Molecular masses were measured by SDS–PAGE and non-denaturing PAGE after silver staining and/or Western blotting. Protein complexes/subunits were detected by 2-dimensional non-denaturing PAGE/SDS–PAGE. A simplified gel shift experiment displayed binding to fragmented calf thymus DNA. Nuclei were richest in poly(malate) binding proteins followed by cytoplasm and membranes. A protein of 370 kDa dissociated into 11 subunits of 11–29 kDa, indicative of a highly complex protein. This and other proteins displayed binding to nucleic acid in gel shift experiments. Poly(malate) is considered a structural and functional equivalent of long contiguous aspartate repeats in proteins of eukaryotes.
|Date:||24 March 2006|
|Institutions:||Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie|
|Keywords:||Physarum polycephalum; Poly(malic acid; Poly(malic acid) binding proteins; Affinity chromatography; Poly(malate)-aminohexyl–Sepharose; Myxomycete; Plasmodium; Nuclear synchrony; Molecular mimicry; Poly(aspartic acid)|
|Subjects:||500 Science > 570 Life sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||14 Jul 2006|
|Last Modified:||20 Jul 2011 20:48|