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Library of prefabricated locked nucleic acid hydrolysis probes facilitates rapid development of reverse-transcription quantitative real-time PCR assays for detection of novel influenza A/H1N1/09 virus.

Wenzel, Jürgen J and Walch, Heiko and Bollwein, Markus and Niller, Hans Helmut and Ankenbauer, Waltraud and Mauritz, Ralf and Höltke, Hans-Joachim and Zepeda, Héctor Manuel and Wolf, Hans and Jilg, Wolfgang and Reischl, Udo (2009) Library of prefabricated locked nucleic acid hydrolysis probes facilitates rapid development of reverse-transcription quantitative real-time PCR assays for detection of novel influenza A/H1N1/09 virus. Clinical chemistry 55 (12), pp. 2218-22.

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Other URL: http://www.clinchem.org/cgi/content/full/55/12/2218


Abstract

BACKGROUND: The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. METHODS: We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLibrary (UPL)--a collection of 165 presynthesized, fluorescence-labeled locked ...

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Item Type:Article
Date:27 November 2009
Institutions:Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:
ValueType
19797710PubMed ID
10.1373/clinchem.2009.136192DOI
Keywords:Influenza A/H1N1/09; Locked Nucleic Acid Hydrolysis Probes; Molecular Detection ; Real-Time RT-PCR; Universal ProbeLibrary
Subjects:500 Science > 570 Life sciences
500 Science > 500 Natural sciences & mathematics
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Dr. Jürgen Wenzel
Deposited On:15 Dec 2009 07:34
Last Modified:13 Mar 2014 12:18
Item ID:11456
Owner Only: item control page

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