Dayoub, Rania and Thasler, Wolfgang E. and Bosserhoff, Anja K. and Singer, Thomas and Jauch, Karl-Walter and Schlitt, Hans J. and Weiss, Thomas S. (2006) Regulation of polyamine synthesis in human hepatocytes by hepatotrophic factor augmenter of liver regeneration. Biochemical and biophysical research communications 345 (1), pp. 181-187.
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Other URL: http://dx.doi.org/10.1016/j.bbrc.2006.04.040
Different stages of liver regeneration are regulated by a variety of factors such as the liver growth associated protein ALR, augmenter of liver regeneration. Furthermore, small molecules like polyamines were proven to be essential for hepatic growth and regeneration. Therefore, using primary human hepatocytes in vitro we investigated the effect of ALR on the biosynthesis of polyamines. We demonstrated by HPLC analysis that recombinant ALR enhanced intracellular hepatic putrescine, spermidine, and spermine levels within 9-12h. The activation of polyamine biosynthesis was dose dependent with putrescine showing the strongest increase. Additionally, ALR treatment induced mRNA expression of ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase, both key enzymes of polyamine biosynthesis. Further, ALR induced c-myc mRNA expression, a regulator of ODC expression, and therefore we assume that ALR exerts its liver regeneration augmenting effects through stimulation of its signalling pathway leading in part to enhanced polyamine synthesis.
|Date:||23 June 2006|
|Institutions:||Medicine > Lehrstuhl für Chirurgie|
|Keywords:||Liver regeneration; Polyamines; Ornithine decarboxylase; Hepatotrophic growth factor; Augmenter of liver regeneration; Immediate early gene; c-myc|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||22 Mar 2007|
|Last Modified:||13 Mar 2014 09:47|