Banas, B. and Eberle, J. and Banas, B. and Schlöndorff, D. and Luckow, B. (2001) Modulation of HIV-1 enhancer activity and virus production by cAMP. FEBS letters 509 (2), pp. 207-212.
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The effect of cAMP on the transcriptional activity of the HIV-1 long terminal repeat/enhancer was investigated and compared to the effect of cAMP on virus replication. In culture cAMP repressed virus replication in vivo using different cell types. Transient transfection studies with HIV-1 enhancer-derived luciferase reporter gene constructs identified the minimal DNA sequence mediating the negative regulatory effect of cAMP on HIV-1 transcription. A single nuclear factor kappaB element from the HIV-1 enhancer mediates the repressive effect on transcription. AP-2 is not involved in cAMP repression. Stable transfection of Jurkat T cells with the co-activators CREB binding protein (CBP) and p300 completely abolished the cAMP repressive effect, supporting the hypothesis that elevation of intracellular cAMP increases phosphorylation of CREB, which then competes with phosphorylated p65 and Ets-1 for limiting amounts of CBP/p300 thereby mediating the observed repressive effect on transcription. These findings suggest an important role of cAMP on HIV-1 transcription.
|Date:||7 December 2001|
|Institutions:||Medicine > Lehrstuhl für Innere Medizin II|
|Keywords:||HIV-1 enhancer; cAMP; Negative regulation; Transient transfection; Virus production|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Deposited On:||12 Mar 2007|
|Last Modified:||20 Jul 2011 20:53|