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Identification and Reduction of Cryoinjury in Endothelial Cells: A First Step toward Establishing a Cell Bank for Vascular Tissue Engineering

Lehle, Karla and Hoenika, Markus and Jacobs, Volker R. and Schmid, Franz X. and Birnbaum, Dietrich E. (2006) Identification and Reduction of Cryoinjury in Endothelial Cells: A First Step toward Establishing a Cell Bank for Vascular Tissue Engineering. Tissue engineering 12 (12), pp. 3439-3447.

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Other URL: http://www.liebertonline.com/doi/pdf/10.1089/ten.2006.12.3439


Abstract

We analyzed a cryopreservation protocol which improves long-term storage of endothelial cells (EC) for tissue engineering purposes. Human umbilical vein EC were frozen in a high-potassium solution containing 10% dimethyl sulfoxide using 3 different cooling rates. After a storage time in liquid nitrogen of 1, 4, or 12 months, samples were thawed and compared to fresh cells in terms of growth ...

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Item Type:Article
Date:December 2006
Institutions:Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Owner: Ute Lange
Deposited On:29 Mar 2007
Last Modified:13 Mar 2014 09:49
Item ID:1292
Owner Only: item control page

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