Pietrzyk, M. C. and Banas, B. and Wolf, K. and Rümmele, P. and Woenckhaus, M. and Hoffmann, U. and Krämer, B. K. and Fischereder, M. (2004) Quantitative gene expression analysis of fractalkine using laser microdissection in biopsies from kidney allografts with acute rejection. Transplantation proceedings 36 (9), pp. 2659-2661.
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Percutaneous biopsy of the kidney remains the gold standard to establish a diagnosis in renal diseases. Only semiquantitative assessments of gene expression on biopsies have been possible so far. We studied gene expression of the chemokine fractalkine (FKN) in 12 biopsies from laser microdissected kidney allografts that showed histologic signs of acute rejection and 10 controls. As quantified by real-time PCR, the relative tubular FKN expression increased from 1.0 [0.81 to 2.95] (median [range]) in controls to 12.44 [0.90 to 191.0] in acute rejection (P < .01); glomerular FKN expression from 1.3 [0.07 to 27.44] to 12.22 [1.32 to 50.23] (P < .05); and vascular expression, from 0.72 [0.37 to 5.11] to 7.07 [1.19 to 73.49] (P < .01). Furthermore, there was a trend toward higher glomerular FKN expression among patients with more severe rejection. Our results suggest a role of FKN in acute renal allograft rejection.
|Institutions:||Medicine > Lehrstuhl für Innere Medizin II|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||16 Mar 2007|
|Last Modified:||20 Jul 2011 20:54|