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Electron tomography of ice-embedded prokaryotic cells

Grimm, R. and Singh, H. and Rachel, Reinhard and Typke, D. and Zillig, W. and Baumeister, W. (1998) Electron tomography of ice-embedded prokaryotic cells. Biophysical journal 74 (2), pp. 1031-1042.

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Abstract

Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm thick, and their structures were reconstructed to a resolution of 20-40 nm from tilt series comprising 50-140 images. The dose was kept within tolerable limits. A resolution of 20 nm allowed ...

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Item Type:Article
Date:1998
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Michael Thomm
Identification Number:
ValueType
7787027PubMed ID
10.1016/S0006-3495(98)74028-7DOI
Classification:
NotationType
AutomationMESH
FreezingMESH
IceMESH
Image Processing, Computer-AssistedMESH
Microscopy, Electron/methodsMESH
Models, StructuralMESH
Sensitivity and SpecificityMESH
Sulfolobus/ultrastructureMESH
Thermoproteaceae/ultrastructureMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:15 Mar 2010 08:39
Last Modified:15 Mar 2010 08:39
Item ID:13300
Owner Only: item control page
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