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Establishing wild-type levels of catalytic activity on natural and artificial (beta alpha)8-barrel protein scaffolds

Claren, Jörg and Malisi, Christoph and Höcker, Birte and Sterner, Reinhard (2009) Establishing wild-type levels of catalytic activity on natural and artificial (beta alpha)8-barrel protein scaffolds. Proceedings of the National Academy of Sciences of the United States of America 106 (10), pp. 3704-9.

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Abstract

The generation of high levels of new catalytic activities on natural and artificial protein scaffolds is a major goal of enzyme engineering. Here, we used random mutagenesis and selection in vivo to establish a sugar isomerisation reaction on both a natural (beta alpha)(8)-barrel enzyme and a catalytically inert chimeric (beta alpha)(8)-barrel scaffold, which was generated by the recombination of ...

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Item Type:Article
Date:2009
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner
Identification Number:
ValueType
7017727PubMed ID
10.1073/pnas.0810342106DOI
Classification:
NotationType
Aldose-Ketose Isomerases/metabolismMESH
Anthranilic Acids/metabolismMESH
Bacterial Proteins/metabolismMESH
BiocatalysisMESH
Catalytic DomainMESH
Crystallography, X-RayMESH
Evolution, MolecularMESH
IsomerismMESH
LigandsMESH
Models, MolecularMESH
Mutant Proteins/metabolismMESH
Protein Structure, SecondaryMESH
Ribosemonophosphates/metabolismMESH
Substrate SpecificityMESH
Thermotoga maritima/enzymologyMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Owner: Gernot Deinzer
Deposited On:19 Mar 2010 11:10
Last Modified:19 Mar 2010 11:10
Item ID:13649
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