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Characterisation of a thermoalkali-stable cyclodextrin glycosyltransferase from the anaerobic thermoalkaliphilic bacterium Anaerobranca gottschalkii.

Thiemann, Volker and Dönges, Catharina and Prowe, Steffen G and Sterner, Reinhard and Antranikian, Garabed (2004) Characterisation of a thermoalkali-stable cyclodextrin glycosyltransferase from the anaerobic thermoalkaliphilic bacterium Anaerobranca gottschalkii. Archives of microbiology 182 (2-3), pp. 226-35.

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Abstract

The thermoalkaliphilic anaerobic bacterium Anaerobranca gottschalkii produces an extracellular CGTase when grown on starch at 55 degrees C and pH 9.0. The gene encoding this CGTase was cloned and successfully expressed in Escherichia coli. It encodes a protein consisting of 721 amino acids with a signal sequence of 34 amino acids. On SDS-polyacrylamide gels, the purified CGTase from A. ...

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Item Type:Article
Date:2004
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner
Identification Number:
ValueType
15340782PubMed ID
10.1007/s00203-004-0717-xDOI
Classification:
NotationType
Amino Acid SequenceMESH
Amylopectin/metabolismMESH
Amylose/metabolismMESH
Bacterial Proteins/physiologyMESH
Calcium Chloride/pharmacologyMESH
Cloning, MolecularMESH
Coenzymes/pharmacologyMESH
Enzyme StabilityMESH
Escherichia coli/metabolismMESH
Gene ExpressionMESH
Genes, BacterialMESH
Glucosyltransferases/metabolismMESH
Gram-Positive Endospore-Forming Rods/enzymologyMESH
Hydrogen-Ion ConcentrationMESH
Molecular Sequence DataMESH
Molecular WeightMESH
Open Reading FramesMESH
Protein Sorting SignalsMESH
Recombinant Proteins/metabolismMESH
Starch/metabolismMESH
Substrate SpecificityMESH
TemperatureMESH
alpha-Cyclodextrins/metabolismMESH
beta-Cyclodextrins/metabolismMESH
gamma-Cyclodextrins/metabolismMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:15 Apr 2010 05:53
Last Modified:15 Apr 2010 05:53
Item ID:13681
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