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Isolation and phenotypic characterization of colonic macrophages

Rogler, G. and Hausmann, M. and Vogl, D. and Aschenbrenner, E. and Andus, T. and Falk, W. and Andreesen, Reinhard and Schölmerich, J. and Gross, V. (1998) Isolation and phenotypic characterization of colonic macrophages. Clinical and experimental immunology 112 (2), pp. 205-15.

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Abstract

Macrophages play an important role in the intestinal mucosal immune system. However, they are a poorly defined cell population. We therefore determined their phenotype in normal colonic mucosa. Macrophages were isolated from colonic biopsies and surgical specimens by collagenase digestion. Colonic macrophages were positively sorted by anti-CD33 magnetic beads. Flow cytometric triple fluorescence ...

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Item Type:Article
Date:1998
Institutions:Medicine > Abteilung für Hämatologie und Internistische Onkologie
Identification Number:
ValueType
6833764PubMed ID
Classification:
NotationType
Antigen-Presenting Cells/immunologyMESH
Antigens, CD/analysisMESH
Antigens, CD86MESH
Antigens, Differentiation, Myelomonocytic/analysisMESH
Biological Markers/analysisMESH
Cell SeparationMESH
Colon/immunologyMESH
Flow CytometryMESH
HumansMESH
Immunity, MucosalMESH
Interleukin-1/secretionMESH
Intestinal Mucosa/immunologyMESH
Macrophages/secretionMESH
Membrane Glycoproteins/analysisMESH
PhenotypeMESH
Receptors, Immunologic/analysisMESH
Th1 Cells/immunologyMESH
Th2 Cells/immunologyMESH
Tumor Necrosis Factor-alpha/secretionMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:15 Apr 2010 05:36
Last Modified:15 Apr 2010 05:36
Item ID:14322
Owner Only: item control page
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