Ewing, Patricia and Wilke, Andrea and Brockhoff, Gero and Andreesen, Reinhard and Eissner, Günther and Holler, Ernst and Gerbitz, Armin (2003) Isolation and transplantation of allogeneic pulmonary endothelium derived from GFP transgenic mice. Journal of immunological methods 283 (1-2), pp. 307-15.
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The isolation of primary endothelial cells from murine tissues has long been a challenge and remains a difficult task. Using GFP transgenic C57/BL6 mice as donors, we describe a reliable method to isolate pulmonary endothelial cells by flow cytometry after staining with DiI-Ac-low density lipoprotein (LDL). After mechanical dissociation of murine lung tissue and enzymatic digestion, adherent ...
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|Institutions:||Medicine > Abteilung für Hämatologie und Internistische Onkologie|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||20 Apr 2010 05:48|
|Last Modified:||20 Apr 2010 05:48|