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Isolation and transplantation of allogeneic pulmonary endothelium derived from GFP transgenic mice

Ewing, Patricia and Wilke, Andrea and Brockhoff, Gero and Andreesen, Reinhard and Eissner, Günther and Holler, Ernst and Gerbitz, Armin (2003) Isolation and transplantation of allogeneic pulmonary endothelium derived from GFP transgenic mice. Journal of immunological methods 283 (1-2), pp. 307-15.

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Abstract

The isolation of primary endothelial cells from murine tissues has long been a challenge and remains a difficult task. Using GFP transgenic C57/BL6 mice as donors, we describe a reliable method to isolate pulmonary endothelial cells by flow cytometry after staining with DiI-Ac-low density lipoprotein (LDL). After mechanical dissociation of murine lung tissue and enzymatic digestion, adherent ...

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Item Type:Article
Date:2003
Institutions:Medicine > Abteilung für Hämatologie und Internistische Onkologie
Identification Number:
ValueType
14659921PubMed ID
Classification:
NotationType
AnimalsMESH
Antigens, CD31/analysisMESH
Cell Separation/methodsMESH
Cells, CulturedMESH
Endothelial Cells/transplantationMESH
FemaleMESH
Green Fluorescent ProteinsMESH
Lipoproteins, LDL/metabolismMESH
Luminescent Proteins/geneticsMESH
Lung/cytologyMESH
MiceMESH
Mice, Inbred C57BLMESH
Mice, TransgenicMESH
Staining and LabelingMESH
Vascular Cell Adhesion Molecule-1/analysisMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:20 Apr 2010 05:48
Last Modified:20 Apr 2010 05:48
Item ID:14419
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