Fischer, Karin and Hoffmann, Petra and Voelkl, Simon and Meidenbauer, Norbert and Ammer, Julia and Edinger, Matthias and Gottfried, Eva and Schwarz, Sabine and Rothe, Gregor and Hoves, Sabine and Renner, Kathrin and Timischl, Birgit and Mackensen, Andreas and Kunz-Schughart, Leoni and Andreesen, Reinhard and Krause, Stefan W. and Kreutz, Marina (2007) Inhibitory effect of tumor cell-derived lactic acid on human T cells. Blood 109 (9), pp. 3812-9.
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A characteristic feature of tumors is high production of lactic acid due to enhanced glycolysis. Here, we show a positive correlation between lactate serum levels and tumor burden in cancer patients and examine the influence of lactic acid on immune functions in vitro. Lactic acid suppressed the proliferation and cytokine production of human cytotoxic T lymphocytes (CTLs) up to 95% and led to a 50% decrease in cytotoxic activity. A 24-hour recovery period in lactic acid-free medium restored CTL function. CTLs infiltrating lactic acid-producing multicellular tumor spheroids showed a reduced cytokine production. Pretreatment of tumor spheroids with an inhibitor of lactic acid production prevented this effect. Activated T cells themselves use glycolysis and rely on the efficient secretion of lactic acid, as its intracellular accumulation disturbs their metabolism. Export by monocarboxylate transporter-1 (MCT-1) depends on a gradient between cytoplasmic and extracellular lactic acid concentrations and consequently, blockade of MCT-1 resulted in impaired CTL function. We conclude that high lactic acid concentrations in the tumor environment block lactic acid export in T cells, thereby disturbing their metabolism and function. These findings suggest that targeting this metabolic pathway in tumors is a promising strategy to enhance tumor immunogenicity.
|Institutions:||Medicine > Abteilung für Hämatologie und Internistische Onkologie|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||20 Apr 2010 11:34|
|Last Modified:||20 Apr 2010 11:34|
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