Kühnel, H. and Briesen, H.v. and Dietrich, U. and Adamski, M. and Mix, D. and Biesert, L. and Kreutz, R. and Immelmann, A. and Henco, K. and Meichnser, C. and Andreesen, Reinhard and Gelderblom, H. and Rübsamen-Waigmann, H. (1989) Molecular cloning of two west african human immunodeficiency virus type 2 isolates which replicate well on macrophages: a gambian isolate from a case of neurologic acquired immunodeficiency syndrome, and highly divergent Ghanesian isolate. Proceedings of the National Academy of Sciences of the United States of America (PNAS) 86 (7), pp. 2383-2287.
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Human immunodeficiency virus type 2 (HIV-2)-related viruses were isolated from a Gambian dying of exclusively neurological disease (HIV-2D194) and from an asymptomatic Ghanian (HIV-2D205). Both strains exhibited properties of HIV-1 biological subtype c: they grew slowly and induced few or no syncytia but eventually produced high levels of particle-associated reverse transcriptase in cultures of fresh peripheral blood lymphocytes, and they established stable infection of T-lymphoma (HUT-78) and monocytic (U937) cell lines. Each produced even higher levels of reverse transcriptase when fresh human monocytes/macrophages were used as target cells. The viruses were molecularly cloned after a single passage in culture, in order to minimize in vitro selection of subtypes present in vivo. Restriction-site analysis showed heterogeneity within each isolate. Nucleotide sequence analysis of a portion of the HIV-2D194 genome revealed that it is a member of the prototypic HIV-2 family, displaying 13% divergence versus HIV-2ROD and HIV-2NIHZ, as compared to 9% divergence between HIV-2ROD and HIV-2NIHZ. In contrast, HIV-2D205 is the most highly divergent HIV-2 strain yet described: it is equidistant in relation between the known HIV-2 strains and the simian immunodeficiency virus isolates from rhesus macaque monkeys (23-25% divergence).
|Institutions:||Medicine > Abteilung für Hämatologie und Internistische Onkologie|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Yes|
|Deposited On:||28 Apr 2010 05:52|
|Last Modified:||28 Apr 2010 05:52|