Mikuz, G. and Hofstaedter, Ferdinand and Delgado, R. (1985) Extraction of cells from paraffin-embedded tissue sections for single-cell DNA cytophotometry. Analytical and quantitative cytology and histology 7 (4), pp. 343-346.
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A simple technique is presented for the isolation of cells from paraffin-embedded tissues for Feulgen DNA cytophotometric investigations. Tissue fragments from paraffin blocks were deparaffinized in xylene, rehydrated and refixed in a formalin solution and incubated in a solution of 0.5 pepsin in 0.25% hydrochloric acid. After filtration through a 70 micron mesh and centrifugation, the cells were ...
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|Institutions:||Medicine > Lehrstuhl für Pathologie|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||05 May 2010 09:27|
|Last Modified:||05 May 2010 09:27|