Mikuz, G. ; Hofstaedter, Ferdinand ; Delgado, R.
Alternative Links zum Volltext:Pubmed
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Analytical and quantitative cytology and histology |
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| Verlag: | Science Printers and Publ. |
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| Band: | 7 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 4 |
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| Seitenbereich: | S. 343-346 |
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| Datum: | 1985 |
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| Institutionen: | Medizin > Lehrstuhl für Pathologie |
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| Identifikationsnummer: | |
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| Klassifikation: | | Notation | Art |
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| Cell Separation/methods | MESH | | Cytophotometry | MESH | | DNA, Neoplasm/analysis | MESH | | Humans | MESH | | Male | MESH | | Neoplasms/pathology | MESH | | Paraffin | MESH | | Pepsin A | MESH | | Sarcoma/pathology | MESH | | Teratoma/pathology | MESH | | Testicular Neoplasms/pathology | MESH | | Urinary Bladder Neoplasms/pathology | MESH |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Unbekannt / Keine Angabe |
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| An der Universität Regensburg entstanden: | Unbekannt / Keine Angabe |
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| Dokumenten-ID: | 14624 |
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Zusammenfassung
A simple technique is presented for the isolation of cells from paraffin-embedded tissues for Feulgen DNA cytophotometric investigations. Tissue fragments from paraffin blocks were deparaffinized in xylene, rehydrated and refixed in a formalin solution and incubated in a solution of 0.5 pepsin in 0.25% hydrochloric acid. After filtration through a 70 micron mesh and centrifugation, the cells were ...
Zusammenfassung
A simple technique is presented for the isolation of cells from paraffin-embedded tissues for Feulgen DNA cytophotometric investigations. Tissue fragments from paraffin blocks were deparaffinized in xylene, rehydrated and refixed in a formalin solution and incubated in a solution of 0.5 pepsin in 0.25% hydrochloric acid. After filtration through a 70 micron mesh and centrifugation, the cells were smeared upon a glass slide. Comparison between the results obtained with freshly prepared imprints and with pepsin-extracted cells of the same tumor showed that the extraction technique does not influence the Feulgen reaction or the DNA distribution pattern. Investigations carried out on bladder and embryonal carcinomas have demonstrated that the method permits an analysis of histologically or histochemically identified tumor cells within individual tissue areas.
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