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Cryopreservation of human endothelial cells for vascular tissue engineering.

Lehle, Karla and Hoenicka, Markus and Jacobs, Volker R and Schmid, Franz X and Birnbaum, Dietrich E (2005) Cryopreservation of human endothelial cells for vascular tissue engineering. Cryobiology 50 (2), pp. 154-61.

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Abstract

To investigate the influence of cryopreservation on endothelial cell growth, morphology, and function human umbilical vein endothelial cells (HUVECs) were frozen following a standard protocol. Cell suspensions were exposed to 10% dimethyl sulfoxide in a high-potassium solution, cooled to -80 degrees C at 1 degrees C/min and stored in liquid nitrogen for 7-36 days. Samples were thawed in a 37 ...

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Item Type:Article
Date:2005
Institutions:Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie
Identification Number:
ValueType
15843005PubMed ID
10.1016/j.cryobiol.2004.12.006DOI
Classification:
NotationType
Cell Adhesion Molecules/biosynthesisMESH
Cell ProliferationMESH
Cryopreservation/methodsMESH
Endothelium, Vascular/cytologyMESH
HumansMESH
Mitochondria/enzymologyMESH
Tetrazolium Salts/metabolismMESH
Thiazoles/metabolismMESH
Thrombomodulin/biosynthesisMESH
Tissue Engineering/methodsMESH
Umbilical Veins/cytologyMESH
Subjects:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Markus Hoenicka
Deposited On:07 Jul 2010 06:46
Last Modified:07 Jul 2010 06:46
Item ID:15653
Owner Only: item control page
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