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Cryopreservation of human endothelial cells for vascular tissue engineering.

Lehle, Karla and Hoenicka, Markus and Jacobs, Volker R. and Schmid, Franz X. and Birnbaum, Dietrich E. (2005) Cryopreservation of human endothelial cells for vascular tissue engineering. Cryobiology 50 (2), pp. 154-61.

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Abstract

To investigate the influence of cryopreservation on endothelial cell growth, morphology, and function human umbilical vein endothelial cells (HUVECs) were frozen following a standard protocol. Cell suspensions were exposed to 10% dimethyl sulfoxide in a high-potassium solution, cooled to -80 degrees C at 1 degrees C/min and stored in liquid nitrogen for 7-36 days. Samples were thawed in a 37 ...

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Item Type:Article
Date:2005
Institutions:Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie
Identification Number:
ValueType
15843005PubMed ID
10.1016/j.cryobiol.2004.12.006DOI
Classification:
NotationType
Cell Adhesion Molecules/biosynthesisMESH
Cell ProliferationMESH
Cryopreservation/methodsMESH
Endothelium, Vascular/cytologyMESH
HumansMESH
Mitochondria/enzymologyMESH
Tetrazolium Salts/metabolismMESH
Thiazoles/metabolismMESH
Thrombomodulin/biosynthesisMESH
Tissue Engineering/methodsMESH
Umbilical Veins/cytologyMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Deposited On:07 Jul 2010 06:46
Last Modified:16 Jan 2015 09:08
Item ID:15653
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