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Identification and reduction of cryoinjury in endothelial cells: a first step toward establishing a cell bank for vascular tissue engineering.

Lehle, Karla and Hoenicka, Markus and Jacobs, Volker R. and Schmid, Franz X. and Birnbaum, Dietrich E. (2006) Identification and reduction of cryoinjury in endothelial cells: a first step toward establishing a cell bank for vascular tissue engineering. Tissue engineering 12 (12), pp. 3439-47.

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Abstract

We analyzed a cryopreservation protocol which improves long-term storage of endothelial cells (EC) for tissue engineering purposes. Human umbilical vein EC were frozen in a high-potassium solution containing 10% dimethyl sulfoxide using 3 different cooling rates. After a storage time in liquid nitrogen of 1, 4, or 12 months, samples were thawed and compared to fresh cells in terms of growth ...

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Item Type:Article
Date:2006
Institutions:Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie
Identification Number:
ValueType
17518680PubMed ID
10.1089/ten.2006.12.3439DOI
Classification:
NotationType
Cell CountMESH
Cell Differentiation/physiologyMESH
Cells, CulturedMESH
CryopreservationMESH
Endothelial Cells/physiologyMESH
Endothelium, Vascular/physiologyMESH
HumansMESH
Tissue BanksMESH
Tissue EngineeringMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Deposited On:07 Jul 2010 06:44
Last Modified:16 Jan 2015 09:07
Item ID:15655
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