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Identification and reduction of cryoinjury in endothelial cells: a first step toward establishing a cell bank for vascular tissue engineering.

Lehle, Karla and Hoenicka, Markus and Jacobs, Volker R and Schmid, Franz X and Birnbaum, Dietrich E (2006) Identification and reduction of cryoinjury in endothelial cells: a first step toward establishing a cell bank for vascular tissue engineering. Tissue engineering 12 (12), pp. 3439-47.

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Abstract

We analyzed a cryopreservation protocol which improves long-term storage of endothelial cells (EC) for tissue engineering purposes. Human umbilical vein EC were frozen in a high-potassium solution containing 10% dimethyl sulfoxide using 3 different cooling rates. After a storage time in liquid nitrogen of 1, 4, or 12 months, samples were thawed and compared to fresh cells in terms of growth ...

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Item Type:Article
Date:2006
Institutions:Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie
Identification Number:
ValueType
17518680PubMed ID
10.1089/ten.2006.12.3439DOI
Classification:
NotationType
Cell CountMESH
Cell Differentiation/physiologyMESH
Cells, CulturedMESH
CryopreservationMESH
Endothelial Cells/physiologyMESH
Endothelium, Vascular/physiologyMESH
HumansMESH
Tissue BanksMESH
Tissue EngineeringMESH
Subjects:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Markus Hoenicka
Deposited On:07 Jul 2010 06:44
Last Modified:07 Jul 2010 06:44
Item ID:15655
Owner Only: item control page
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