Falk, Werner and Goodwin, R.H. and Leonard, E.J.
A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration.
Journal of Immunological Methods 33 (3), pp. 239-247.
We designed a 48-well chemotaxis chamber to minimize manipulation time and amount of material required by the larger blindwell or Boyden chemotaxis chamber. Cell and chemoattractant dose-response curves showed that results were comparable to our better than those obtained with blindwell chambers. The volume of chemoattractant per well is 25 microliter; the number of cells can be as low as 10,000. The time needed for setting up this multiwell unit and for staining the membrane filter sheet is negligible. Combined with the use of an image analyzer to count the number of migrated cells, the method is suitable for clinical research on the functional state of monocytes in large groups of patients.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
|Dose-Response Relationship, Immunologic||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||20 Jul 2010 05:07|
|Last Modified:||20 Jul 2011 22:33|