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Rapid quantitation of neutrophil chemotaxis: use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assembly

Harvath, L. and Falk, Werner and Leonard, E.J. (1980) Rapid quantitation of neutrophil chemotaxis: use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assembly. Journal of immunological methods 37 (1), pp. 39-45.

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Abstract

A neutrophil chemotaxis assay was developd which permits rapid, quantitative assessment of migration across a membrane filter. The critical factor in the assay was the use of a 10 microns thick polycarbonate membrane without the usual polyvinylpyrrolidone coating. Migrated neutrophils remain adherent to the uncoated membrane, whereas 20-50% fall off polyvinylpyrrolidone-coated membranes. A major ...

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Item Type:Article
Date:1980
Institutions:Medicine > Lehrstuhl für Innere Medizin I
Identification Number:
ValueType
7000913PubMed ID
Classification:
NotationType
CarbonatesMESH
Chemotactic Factors/pharmacologyMESH
Chemotaxis, LeukocyteMESH
Dose-Response Relationship, ImmunologicMESH
HumansMESH
Immunologic TechniquesMESH
MembranesMESH
NeutrophilsMESH
PovidoneMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Owner: Universitätsbibliothek Regensburg
Deposited On:20 Jul 2010 05:06
Last Modified:13 Mar 2014 13:53
Item ID:15956
Owner Only: item control page
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