Falk, Werner and Leonard, E.J.
Specificity and reversibility of chemotactic deactivation of human monocytes.
Infection and immunity 32 (2), pp. 464-468.
The chemotactic deactivation of human monocytes was studied to provide insight into the mechanism of chemotaxis. Deactivation was dependent on the dose of chemoattractant and time of incubation. A concentration in the cell suspension of 10(-8) M N-formylmethionylleucyl phenylalanine (FMLP) for 45 min at 37 degrees C led to 60% suppression of the subsequent specific chemotactic response. Higher concentrations of FMLP led to almost 100% specific suppression. Deactivation was specific under all conditions used. The response to a nonrelated chemoattractant, human serum-derived C5a, was unaffected by incubation in FMLP. Deactivation was also transient. If cells were deactivated at 37 degrees C with FMLP, they recovered within 6 h at 37 degrees C from this deactivation. Both phenomena, deactivation and recovery from deactivation, were temperature dependent. Monocytes could not be deactivated at 0 degrees C, and they did not recover from deactivation when kept at 0 degrees C. Thus, specific deactivation appears to require cellular metabolism, involving loss of receptors or blocking of a step between receptor occupancy and response.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
|Chemotaxis, Leukocyte/drug effects||MESH|
|Dose-Response Relationship, Drug||MESH|
|Methionine/analogs & derivatives||MESH|
|Receptors, Cell Surface/metabolism||MESH|
|Receptors, Formyl Peptide||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||20 Jul 2010 06:14|
|Last Modified:||20 Jul 2011 22:33|