Männel, Daniela N. and Falk, Werner
Interferon-gamma is required in activation of macrophages for tumor cytotoxicity.
Cellular immunology 79 (2), pp. 396-402.
The participation of interferon-gamma in activation of murine macrophages for tumor cell lysis was investigated. Biochemically macrophage activation factor and interferon-gamma have not been separated. Antiviral titers correlated closely with macrophage activation in antigen- or mitogen-induced spleen cell supernatants. A monoclonal rat antibody that neutralized virus-induced interferon was also found to neutralize interferon-gamma in such supernatants. These monoclonal antibodies were coupled to CH-Sepharose 4B and used for absorption of antiviral activity from mitogen-induced spleen cell supernatants. Absorption of the interferon was paralleled by the reduction of the macrophage-activating capacity of the supernatants. Data from control absorptions supported the specificity of the absorption effect. These results indicate that interferon-gamma is required for activation of macrophages for tumor cell lysis. These results can be interpreted in two ways: (a) the monoclonal antibodies cross-react with interferon-gamma and with a mediator that is required for activation of macrophages for tumor cell lysis or (b) interferon-gamma itself is an essential cofactor for macrophage activation.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
Medicine > Lehrstuhl für Immunologie
|Mice, Inbred C3H||MESH|
|Rats, Inbred Strains||MESH|
|Vesicular stomatitis Indiana virus/growth & development||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||21 Jul 2010 05:06|
|Last Modified:||20 Jul 2011 22:33|