Dröge, W. and Männel, D.N. and Falk, Werner and Lehmann, V. and Schmidt, H. and Nick, S. and Häcker-Shahin, B. and Jänicke, R.
Suppression of cytotoxic T lymphocyte activation by L-ornithine.
Journal of immunology (Baltimore, Md. : 1950) 134 (5), pp. 3379-3383.
The effect of L-ornithine on several types of immune reactions was analyzed. L-ornithine was found to suppress the activation of cytotoxic T lymphocytes (CTL) in vivo and in vitro. This suppressive effect was not observed with the structural analogues D-ornithine, L-lysine, or putrescine or with the amino acids L-histidine or L-alanine. The concentration of 9 X 10(-3) M L-ornithine was found to mediate a practically complete suppression of the cytotoxic response in vitro if applied on day 0 or day 1 of the culture, but a comparably weak suppression if applied on day 3. The same concentration of L-ornithine had no effect on the production of the lymphokines interleukin 2 (IL 2) and gamma-interferon (IFN-gamma). This concentration of ornithine had also no substantial effect on several types of proliferative responses, including the allogeneic mixed lymphocyte reaction, the concanavalin A-activated IL 2-dependent proliferation of thymocytes, and IL 2-dependent proliferation of the T cell clone W-2. These observations suggest that L-ornithine inhibits selectively the differentiation of CTL effector cells. By the criteria tested, the immunosuppressive effect of L-ornithine is more selective than that of cyclosporine A, which was previously found to suppress not only the activation of cytotoxic activity but also proliferative responses and the production of the lymphokines IL 2 and IFN-gamma.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
|Cytotoxicity, Immunologic/drug effects||MESH|
|Lymphocyte Activation/drug effects||MESH|
|Mice, Inbred C3H||MESH|
|Mice, Inbred C57BL||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||21 Jul 2010 05:10|
|Last Modified:||20 Jul 2011 22:33|