Falk, Werner and Männel, D.N. and Katzer, B. and Kaltmann, B. and Krammer, P.H. and Diamantstein, T. and Dröge, W.
Induction of IL 2 receptor expression and cytotoxicity of thymocytes by stimulation with TCF1.
Journal of immunology (Baltimore, Md. : 1950) 135 (2), pp. 1160-1164.
We investigated the role of T cell cytotoxicity inducing factor 1 (TCF1) in the induction of a cytotoxic T cell response. We found that help-deficient thymocyte cultures supplied with saturating amounts of purified IL 2 did not develop CTL in a 5-day culture. The expression of cytotoxicity was dependent on the addition of TCF1 derived from the T cell hybridoma K15. TCF1 also induced proliferation of thymocytes in the presence of IL 2. Only the PNA- thymocyte subpopulation responded to TCF1 with proliferation and cytotoxicity in the presence of IL 2. The monokine IL 1 also induced proliferation in this subpopulation but failed to induce cytotoxicity. IL 1 was further distinguished from TCF1 by inhibition of IL 1-induced but not TCF1-induced proliferation by anti-IL 1 antibodies. In addition, using anti-IL 2 receptor antibodies (AMT 13), we showed that TCF1 in the presence of IL 2 substantially increased IL 2 receptor expression in thymocytes. IL 1 had the same effect on induction of IL 2 receptor expression as TCF1. Because some effects of IL 1 and TCF1 are distinct and some overlap, we discuss whether IL 1 and TCF1 induce different subsets of PNA- thymocytes.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
|Mice, Inbred C3H||MESH|
|Suppressor Factors, Immunologic||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||21 Jul 2010 05:11|
|Last Modified:||20 Jul 2011 22:33|