Männel, D.N. and Mizel, S.B. and Diamantstein, T. and Falk, Werner
Induction of interleukin 2 responsiveness in thymocytes by synergistic action of interleukin 1 and interleukin 2.
Journal of immunology (Baltimore, Md. : 1950) 134 (5), pp. 3108-3110.
Thymocyte cultures from C3H/HeJ mice were stimulated for proliferative responses with purified preparations of interleukin 1 (IL 1) and interleukin 2 (IL 2). Synergistic responses were obtained in the absence of mitogen. In the presence of excess IL 2, the thymocyte proliferation response was strictly dependent on the amount of IL 1 in the cultures. Antibodies to IL 1 inhibited the response in a dose-dependent manner. The combination of IL 1 plus IL 2 induced the appearance of IL 2 receptors on murine thymocytes as detected with a monoclonal antibody directed against the IL 2 receptor. Neither IL 1 nor IL 2 alone had this effect. The thymic subpopulation found to become IL 2 responsive upon IL 1 stimulus was the peanut agglutinin-negative (PNA-) medullary fraction.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
|Mice, Inbred C3H||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||21 Jul 2010 09:23|
|Last Modified:||20 Jul 2011 22:33|