Falk, Werner and Krammer, P.H. and Männel, D.N.
A new assay for interleukin-1 in the presence of interleukin-2.
Journal of immunological methods 99 (1), pp. 47-52.
A simple and reliable assay for interleukin-1 (IL-1) is described which has the advantage over other assays that it is independent of interleukin-2 (IL-2) production by the test cells. The assay makes possible the detection of IL-1 in the supernatants of T cell populations. The ability of IL-1 to induce IL-2 receptor expression in the absence of T cell mitogen is the basis of this assay. Thus, proliferation of mouse thymocytes incubated in the presence of saturating concentrations of IL-2 (10 U/ml) was directly dependent on the concentration of IL-1. The sensitivity of the assay is comparable to the sensitivity of the classical thymocyte co-stimulator assay. Natural and recombinant human and murine IL-1 were measured in this test system with comparable sensitivity.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
|Dose-Response Relationship, Drug||MESH|
|Lymphocyte Activation/drug effects||MESH|
|Mice, Inbred Strains||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||21 Jul 2010 09:21|
|Last Modified:||20 Jul 2011 22:34|