Falk, Werner and Männel, D.N. and Darjes, H. and Krammer, P.H.
IL-1 induces high affinity IL-2 receptor expression of CD4-8- thymocytes.
Journal of immunology (Baltimore, Md. : 1950) 143 (2), pp. 513-517.
We investigated the role of cytokines for the growth of CD4-8-thymocytes (double negative thymocytes) (DNT) in vitro and found that IL-1-induced IL-2-dependent proliferation of only the IL-2R-positive DNT subpopulation. The presence of IL-1 during the first 18 h of culture was sufficient for an optimal response and suggested that IL-1 induced DNT differentiation. We could indeed show by RNA dot blot analysis that IL-1 stimulated de novo expression of the p55 chain of the IL-2R thus initiating high affinity IL-2 binding and a proliferative response. Because macrophages and epithelial cells in the thymus produce IL-1 we propose that IL-1 is involved in early events during maturation of immature thymocytes.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
|Antigens, Differentiation, T-Lymphocyte||MESH|
|Mice, Inbred C3H||MESH|
|Nucleic Acid Hybridization||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||22 Jul 2010 05:00|
|Last Modified:||20 Jul 2011 22:34|