Rupprecht, M. and Rupprecht, R. and Wodarz, Norbert and Braner, H.-U. and Kornhuber, J. and Koch, H. U. and Riederer, P. and Hornstein, O. P.
In vivo and in vitro effects of glucocorticoids on lectin-induced blastogenesis in atopic dermatitis.
Archives of dermatological research 283 (5), pp. 292-296.
The effects of glucocorticoids administered in vivo and in vitro on lectin-induced proliferation of lymphocytes sampled from venous blood were investigated in patients with atopic dermatitis (AD) and in normal controls. Stimulation by concanavalin A (Con A), phytohaemagglutinin A (PHA) and pokeweed mitogen (PWM) in patients and controls did not differ significantly under baseline conditions. After in vivo administration of methylprednisolone the decline of Con A-induced blastogenesis of leucocytes was similar in both groups, whereas PHA stimulation caused a significant reduction in the controls only. In vitro addition of different dexamethasone concentrations had a pronounced suppressive effect on Con A- and PHA-induced blastogenesis in both groups, whereas PWM stimulation was unaffected. Pretreatment in vivo with methylprednisolone further decreased the suppression of the Con A and PHA lymphocyte proliferation rate by dexamethasone added in vitro in controls but not in patients. With regard to B-cell proliferation generated by PWM, no consistent glucocorticoid effect could be observed. The impaired effect on lymphocyte blastogenesis of glucocorticoids administered in vivo, in contrast to a normal in vitro reaction to dexamethasone, together with recent findings of an altered glucocorticoid receptor pharmacology in AD, points to a decreased biological in vivo efficiency of methylprednisolone in atopic dermatitis.
|Institutions:|| Medicine > Lehrstuhl für Psychiatrie und Psychotherapie|
|Lymphocyte Activation/drug effects||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||27 Aug 2010 12:14|
|Last Modified:||20 Jul 2011 22:35|