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Characterisation of the metal-ion-GDP complex at the active sites of transforming and nontransforming p21 proteins by observation of the ¹⁷O-Mn superhyperfine coupling and by kinetic methods

Feuerstein, J. and Kalbitzer, Hans Robert and John, J. and Goody, R. S. and Wittinghofer, A. (1987) Characterisation of the metal-ion-GDP complex at the active sites of transforming and nontransforming p21 proteins by observation of the ¹⁷O-Mn superhyperfine coupling and by kinetic methods. European journal of biochemistry (= the FEBS journal) 162 (1), pp. 49-55.

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Abstract

Kinetic studies on the interaction of three Ha-ras-encoded p21 proteins with GDP and MgGDP have yielded values for the association (10(6)-10(7) M-1 s-1) and dissociation (10(-3)-10(-5) s-1) rate constants at 0 degrees C. Dramatic differences in the rate constants were not observed for the three proteins. Under non-physiological conditions (absence of Mg2+), the rate constant for GDP release was ...

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Item Type:Article
Date:1987
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
3028791PubMed ID
10.1111/j.1432-1033.1987.tb10540.xDOI
Classification:
NotationType
Binding SitesMESH
Electron Spin Resonance SpectroscopyMESH
Guanine Nucleotides/metabolismMESH
Guanosine Diphosphate/metabolismMESH
KineticsMESH
Magnesium/metabolismMESH
Manganese/metabolismMESH
Oxygen IsotopesMESH
Protein BindingMESH
Proto-Oncogene Proteins/metabolismMESH
Proto-Oncogene Proteins p21(ras)MESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:03 Sep 2010 12:42
Last Modified:03 Sep 2010 12:42
Item ID:16440
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