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Enzyme IIIlac of the staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: site-specific mutagenesis of histidine residues, biochemical characterization and ¹H-NMR studies.

Finkeldei, U. and Kalbitzer, Hans Robert and Eisermann, R. and Stewart, G. C. and Hengstenberg, W. (1991) Enzyme IIIlac of the staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: site-specific mutagenesis of histidine residues, biochemical characterization and ¹H-NMR studies. Protein engineering 4 (4), pp. 469-473.

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Abstract

The lactose-specific phosphocarrier protein enzyme III of the bacterial phosphoenol-pyruvate-dependent phosphotransferase system of Staphylococcus aureus was modified by site-specific mutagenesis on the corresponding lacF gene in order to replace the histidine residues 78 and 82 of the amino acid sequence with a serine residue. Wild-type and both mutant genes were overexpressed in Escherichia ...

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Item Type:Article
Date:1991
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
1881873PubMed ID
Classification:
NotationType
Bacterial Proteins/geneticsMESH
Base SequenceMESH
Binding SitesMESH
Genes, BacterialMESH
HistidineMESH
Magnetic Resonance SpectroscopyMESH
Molecular Sequence DataMESH
Mutagenesis, Site-DirectedMESH
Phosphoenolpyruvate Sugar Phosphotransferase System/geneticsMESH
Phosphoproteins/geneticsMESH
PhosphorylationMESH
Protein EngineeringMESH
Protein Processing, Post-TranslationalMESH
Recombinant Fusion Proteins/chemistryMESH
Staphylococcus aureus/geneticsMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:07 Sep 2010 06:20
Last Modified:07 Sep 2010 06:20
Item ID:16459
Owner Only: item control page
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