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Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product

Wolber, V. and Rensland, H. and Brandmeier, B. and Sagemann, M. and Hoffmann, R. and Kalbitzer, Hans Robert and Wittinghofer, A. (1992) Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product. European journal of biochemistry: EJB (= The FEBS journal) 205 (3), pp. 1115-1121.

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Abstract

The human immunodeficiency virus 1 (HIV-1) nef gene encoded by the HIV-1 isolate lymphadenopathy-associated virus type 1 was expressed in Escherichia coli under the control of the tac promoter. The protein is found mainly in the soluble part of the bacterial lysate; a simple two-column purification scheme has been developed allowing isolation of the recombinant protein without using denaturing ...

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Item Type:Article
Date:1992
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
1533585PubMed ID
Classification:
NotationType
Adenine Nucleotides/metabolismMESH
Adenosine Triphosphatases/metabolismMESH
Base SequenceMESH
Chromatography, High Pressure LiquidMESH
Circular DichroismMESH
Electrophoresis, Polyacrylamide GelMESH
Escherichia coli/geneticsMESH
GTP Phosphohydrolases/metabolismMESH
GTP-Binding Proteins/metabolismMESH
Gene ExpressionMESH
Gene Products, nef/metabolismMESH
Genes, BacterialMESH
Guanine Nucleotides/metabolismMESH
HIV-1/metabolismMESH
Molecular Sequence DataMESH
PhosphorylationMESH
PlasmidsMESH
Promoter Regions, GeneticMESH
Recombinant Proteins/metabolismMESH
Spectrophotometry, UltravioletMESH
nef Gene Products, Human Immunodeficiency VirusMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:07 Sep 2010 06:14
Last Modified:07 Sep 2010 06:14
Item ID:16465
Owner Only: item control page
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