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Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product

Wolber, V. and Rensland, H. and Brandmeier, B. and Sagemann, M. and Hoffmann, R. and Kalbitzer, Hans Robert and Wittinghofer, A. (1992) Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product. European journal of biochemistry: EJB (= The FEBS journal) 205 (3), pp. 1115-1121.

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The human immunodeficiency virus 1 (HIV-1) nef gene encoded by the HIV-1 isolate lymphadenopathy-associated virus type 1 was expressed in Escherichia coli under the control of the tac promoter. The protein is found mainly in the soluble part of the bacterial lysate; a simple two-column purification scheme has been developed allowing isolation of the recombinant protein without using denaturing ...


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Item Type:Article
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
1533585PubMed ID
Adenine Nucleotides/metabolismMESH
Adenosine Triphosphatases/metabolismMESH
Base SequenceMESH
Chromatography, High Pressure LiquidMESH
Circular DichroismMESH
Electrophoresis, Polyacrylamide GelMESH
Escherichia coli/geneticsMESH
GTP Phosphohydrolases/metabolismMESH
GTP-Binding Proteins/metabolismMESH
Gene ExpressionMESH
Gene Products, nef/metabolismMESH
Genes, BacterialMESH
Guanine Nucleotides/metabolismMESH
Molecular Sequence DataMESH
Promoter Regions, GeneticMESH
Recombinant Proteins/metabolismMESH
Spectrophotometry, UltravioletMESH
nef Gene Products, Human Immunodeficiency VirusMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
Created at the University of Regensburg:Unknown
Deposited On:07 Sep 2010 06:14
Last Modified:07 Sep 2010 06:14
Item ID:16465
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