Männel, D. N. and Jänicke, R. and Westenfelder, U. and Echtenacher, B. and Kist, A. and Falk, Werner
Tumor-induced tumor necrosis factor production in macrophages.
Lymphokine research 9 (4), pp. 485-489.
Tumor-associated tumor necrosis factor (TNF) production in patients as well as a TNF-inducing membrane constituent of tumor cells have been reported. In a murine fibrosarcoma model we analyzed TNF production during growth of a tumor transplant. In situ hybridization showed that a gradually increasing number of cells within the tumor tissue became positive for TNFmRNA. Also, in spleen cells of tumor-bearing mice TNFmRNA became more abundant in later stages of tumor growth compared to early stages. In plasma of these animals, however, TNF activity was not detected at any time even after stimulation with bacterial endotoxin. Neutralization with monoclonal antibodies of endogenous TNF during tumor growth did not affect the growth rate of the tumor, indicating that either the antibodies did not reach the relevant TNF production and action sites or that endogenously produced TNF did not play a significant role in this tumor model.
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
|Enzyme-Linked Immunosorbent Assay||MESH|
|Mice, Inbred C3H||MESH|
|Nucleic Acid Hybridization||MESH|
|Tumor Necrosis Factor-alpha/biosynthesis||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||08 Sep 2010 05:21|
|Last Modified:||20 Jul 2011 22:36|