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Mobile segments in rabbit skeletal muscle F-actin detected by ¹H nuclear magnetic resonance spectroscopy

Slósarek, G. ; Heintz, D. ; Kalbitzer, Hans Robert



Zusammenfassung

Polymerization of actin by increasing the ionic strength leads to a quenching of almost all 1H NMR signals. Surprisingly, distinct signals with relatively small line widths can still be observed in actin filaments (F-actin) indicating the existence of mobile, NMR visible residues in the macromolecular structure. The intensity of the F-actin spectrum is much reduced if one replaces Mg2+ with Ca2+, ...

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