Freund, J. and Vértesy, L. and Koller, K. P. and Wolber, V. and Heintz, D. and Kalbitzer, Hans Robert (1995) Complete ¹H nuclear magnetic resonance assignments and structural characterization of a fusion protein of the alpha-amylase inhibitor tendamistat with the activation domain of the human immunodeficiency virus type 1 Tat protein. Journal of molecular biology: JMB 250 (5), pp. 672-688.
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Complete sequence-specific assignments of the1H-NMR spectrum of a fusion protein of the α-amylase inhibitor tendamistat fromStreptomyces tendaeand the activation domain of Tat from human immunodeficiency virus type 1 (HIV-1) was obtained by homonuclear two-dimensional NMR methods. The protein behaves as expected for an ideal fusion protein: the flexible linker allows an almost completely decoupled motion of the subunits of the protein and the two subunits show almost no mutual interaction. In the tendamistat part, small structural distortions due to exchange of the carboxy-terminal leucine propagate mainlyviathe hydrogen bonds of the β-sheet and the disulfide bond. The Tat part of the protein contains the seven cysteine residues of full-length Tat. The fusion protein was expressed inStreptomyces lividansand exported. During the export to the extracellular space disulfide bonds are created by the expressing cells, only one sulfhydryl group remains accessible for sulfhydryl reagents. Although a unique, dominant conformation with a specific disulfide bonding pattern exists, a significant conformational variation can be observed includingcis-proline peptide bonds, which may indicate smaller populations with alternative disulfide bonding patterns.
|Institutions:||Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer|
|Subjects:||500 Science > 570 Life sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||08 Sep 2010 08:02|
|Last Modified:||08 Sep 2010 08:02|