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¹⁵N and ¹H NMR study of histidine containing protein (HPr) from Staphylococcus carnosus at high pressure

Kalbitzer, Hans Robert and Görler, A. and Li, H and Dubovskii, P. V. and Hengstenberg, W. and Kowolik, C. and Yamada, H. and Akasaka, K. (2000) ¹⁵N and ¹H NMR study of histidine containing protein (HPr) from Staphylococcus carnosus at high pressure. Protein science 9 (4), pp. 693-703.

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Abstract

The pressure-induced changes in 15N enriched HPr from Staphylococcus carnosus were investigated by two-dimensional (2D) heteronuclear NMR spectroscopy at pressures ranging from atmospheric pressure up to 200 MPa. The NMR experiments allowed the simultaneous observation of the backbone and side-chain amide protons and nitrogens. Most of the resonances shift downfield with increasing pressure ...

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Item Type:Article
Date:2000
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
1066687PubMed ID
10.1110/ps.9.4.693DOI
Classification:
NotationType
Amides/chemistryMESH
Atmospheric PressureMESH
Bacterial Proteins/chemistryMESH
Magnetic Resonance SpectroscopyMESH
Models, MolecularMESH
Nitrogen IsotopesMESH
Phosphoenolpyruvate Sugar Phosphotransferase System/chemistryMESH
Protein ConformationMESH
ProtonsMESH
Staphylococcus/chemistryMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:15 Sep 2010 08:59
Last Modified:15 Sep 2010 08:59
Item ID:16570
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