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Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPγNH₂ and GTPγF

Stumber, Michael and Herrmann, Christian and Wohlgemuth, Sabine and Kalbitzer, Hans Robert and Jahn, Werner and Geyer, Matthias (2002) Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPγNH₂ and GTPγF. European journal of biochemistry (= the FEBS journal) 269 (13), pp. 3270-3278.

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Abstract

Guanosine triphosphate nucleotide analogues such as GppNHp (also named GMPPNP) or GTPgammaS are widely used to stabilize rapidly hydrolyzing protein-nucleotide complexes and to investigate biochemical reaction pathways. Here we describe the chemical synthesis of guanosine 5'-O-(gamma-amidotriphosphate) (GTPgammaNH(2)) and a new synthesis of guanosine 5'-O-(gamma-fluorotriphosphate) (GTPgammaF). ...

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Item Type:Article
Date:2002
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
12084068PubMed ID
10.1046/j.1432-1033.2002.03003.xDOI
Classification:
NotationType
Biochemistry/methodsMESH
Calorimetry/methodsMESH
Guanosine Triphosphate/metabolismMESH
HydrolysisMESH
Magnesium/metabolismMESH
Magnetic Resonance SpectroscopyMESH
Metals/metabolismMESH
Proto-Oncogene Proteins c-raf/metabolismMESH
ras Proteins/metabolismMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:15 Sep 2010 08:54
Last Modified:15 Sep 2010 08:54
Item ID:16583
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