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NMR-spectroscopic mapping of an engineered cavity in the I14A mutant of HPr from Staphylococcus carnosus using xenon

Gröger, Christian and Möglich, Andreas and Pons, Miguel and Koch, Brigitte and Hengstenberg, Wolfgang and Kalbitzer, Hans Robert and Brunner, Eike (2003) NMR-spectroscopic mapping of an engineered cavity in the I14A mutant of HPr from Staphylococcus carnosus using xenon. Journal of the American Chemical Society 125 (29), pp. 8726-8727.

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Abstract

The interaction between the histidine-containing phosphocarrier protein HPr and xenon atoms in solution is studied in the present paper. Wild-type HPr as well as the exchange mutant I14A have been studied. Specific binding of xenon into an engineered cavity created via the exchange of amino acid residue I14 by alanine could be shown using 1H-15N heteronuclear single-quantum coherence (HSQC) ...

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Item Type:Article
Date:2003
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
12862458PubMed ID
10.1021/ja030113tDOI
Classification:
NotationType
Alanine/chemistryMESH
Amino Acid SubstitutionMESH
Bacterial ProteinsMESH
HydrophobicityMESH
Isoleucine/chemistryMESH
Nitrogen IsotopesMESH
Nuclear Magnetic Resonance, Biomolecular/methodsMESH
Phosphoenolpyruvate Sugar Phosphotransferase System/geneticsMESH
Protein EngineeringMESH
ProtonsMESH
Staphylococcus/chemistryMESH
Xenon/chemistryMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:16 Sep 2010 09:09
Last Modified:16 Sep 2010 09:09
Item ID:16598
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