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Infrequent cavity-forming fluctuations in HPr from Staphylococcus carnosus revealed by pressure- and temperature-dependent tyrosine ring flips

Hattori, Mineyuki ; Li, Hua ; Yamada, Hiroaki ; Akasaka, Kazuyuki ; Hengstenberg, Wolfgang ; Gronwald, Wolfram ; Kalbitzer, Hans Robert



Zusammenfassung

Infrequent structural fluctuations of a globular protein is seldom detected and studied in detail. One tyrosine ring of HPr from Staphylococcus carnosus, an 88-residue phosphocarrier protein with no disulfide bonds, undergoes a very slow ring flip, the pressure and temperature dependence of which is studied in detail using the on-line cell high-pressure nuclear magnetic resonance technique in the ...

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